DNA and serum from sufferers were stored. In addition, control samples from the index individual and family were collected. Standard laboratory assays were used to assess the activity of the classical, MBL, and substitute complement pathways; MBL and ficolin-2 amounts; leukocyte count; distribution of lymphocyte surface area markers; and lymphocyte proliferation in response to mitogens and microbial antigens. Vaccination responses and measurements of immunoglobulin classes and subclasses were obtained from medical information.org.For genomics, the splicing analysis in GeneSpring GX 11.5 has been greatly extended and enhanced to support the new Agilent exon array platform. Agilent SurePrint G3 Exon Microarrays enable experts to identify both exon-level and gene-level expression changes in a single experiment, to capture subtle but essential biological adjustments. Agilent’s GeneSpring GX 11.5 bioinformatics system enhances productivity by enabling investigators to analyze the info at the gene level and at the exon splicing level simultaneously to understand complicated gene expression behavior in a biological context.